產(chǎn)品介紹

Biocolor 硫酸氨基聚糖檢測(cè)試劑盒 
Biocolor Blyscan™ Glycosaminoglycan Assay
 
    采用常規(guī)生化方法，通過標(biāo)準(zhǔn)曲線和比色，可定量分析總硫酸化多糖（氨基聚糖和蛋白聚糖）的含量，亦可測(cè)定O端和N端硫酸氨基多糖的比值。主要采用的是Blyscan染料（Biocolor公司的產(chǎn)品），它可以特異性的與氨基聚糖和蛋白聚糖上的硫酸多糖組分結(jié)合。該方法不適于檢測(cè)已經(jīng)降解的硫酸化雙糖片段。
    該試劑盒可檢測(cè)廣泛的蛋白多糖，可檢測(cè)的氨基多糖包括硫酸軟骨素、硫酸角質(zhì)素、硫酸皮膚素、硫酸肝素，不適合檢測(cè)包含海藻酸的樣品，因?yàn)樗鼈兒刑侨┧帷Ｔ撛噭┖幸膊荒軐?duì)透明質(zhì)酸定量，但透明質(zhì)酸并不干擾對(duì)硫酸化氨基多糖的檢測(cè)。細(xì)胞培養(yǎng)基中20%的胎牛血清也不會(huì)干擾實(shí)驗(yàn)。該方法檢測(cè)靈敏度為5ug，所需時(shí)間為1小時(shí)。
 
檢測(cè)樣本：可來自軟骨組織、動(dòng)脈組織、肺組織、皮膚組織、腫瘤組織、細(xì)胞核組織提取物、細(xì)胞培養(yǎng)液、羊水和尿液
 
實(shí)驗(yàn)操作 
1. 繪制標(biāo)準(zhǔn)曲線
2. 待測(cè)樣品的處理：
取50ul待測(cè)樣品進(jìn)行實(shí)驗(yàn)。如果反應(yīng)物在656nm初的吸收峰大于1.5，則對(duì)樣品進(jìn)行稀釋，待測(cè)樣樣品濃度落入標(biāo)準(zhǔn)曲線中段時(shí)，準(zhǔn)確度較高。
3. 硫酸氨基聚糖—染料復(fù)合物的獲得
加1.0ml的Blyscan染料溶液到待測(cè)樣本中，充分混勻，待不溶物出現(xiàn)，離心，棄上清，則獲得紫色的硫酸氨基聚糖—染料復(fù)合物
4. 硫酸氨基聚糖—染料復(fù)合物中染料的釋放
加入1.0ml的染料釋放劑，充分混勻。溶解5min以上，使染料充分釋放
5. 測(cè)定
用分光光度計(jì)于波長656nm處測(cè)定，或使用colorimeter紅色濾光片測(cè)定
 
產(chǎn)品訂購

   Product Code: B1000     110 assays 
   Product Code: B3000     440 assays

參考文獻(xiàn)
 1
Almeida, H. V., Liu, Y., Cunniffe, G. M., Mulhall, K. J., Matsiko, A., Buckley, C. T., O’Brien, F. J. and Kelly, D. J.
(2014) Controlled release of TGF-β3 from cartilage extra cellular matrix derived scaffolds to promote chondrogenesis of
human joint tissue derived stem cells. Acta Biomaterialia.
2
Amin, H. D., Brady, M. A., St-Pierre, J.-P., Stevens, M. M., Overby, D. R. and Ethier, C. R. (2014) Stimulation of
Chondrogenic Differentiation of Adult Human Bone Marrow-Derived Stromal Cells by a Moderate-Strength Static
Magnetic Field. Tissue Engineering Part A.
3
Anilkumar, T. V., Vineetha, V. P., Revi, D., Muhamed, J. and Rajan, A. (2014) Biomaterial properties of cholecystderived scaffold recovered by a non-detergent/enzymatic method. Journal of Biomedical Materials Research Part B:
Applied Biomaterials.
4
Baiguera, S., Del Gaudio, C., Kuevda, E., Gonfiotti, A., Bianco, A. and Macchiarini, P. (2014) Dynamic decellularization
and cross-linking of rat tracheal matrix. Biomaterials 35, 6344–6350.
5
Baiguera, S., Del Gaudio, C., Lucali, E., Kuevda, E., Boieri, M., Mazzanti, B., Bianco, A. and Macchiarini, P. (2014)
Electrospun gelatin scaffolds incorporating rat decellularized brain extracellular matrix for neural tissue engineering.
Biomaterials 35, 1205–1214.
6
Bhattacharyya, S., Feferman, L. and Tobacman, J. K. (2014) Increased Expression of Colonic Wnt9A through Sp1-
Mediated Transcriptional Effects involving Arylsulfatase B, Chondroitin-4-Sulfate, and Galectin-3. Journal of Biological
Chemistry jbc–M114.
7
BUCKLEY, C. T. (2014) Shape-memory porous alginate scaffolds for regeneration of the annulus fibrosus-Effect of TGF-?
3 supplementation and oxygen culture conditions.
8
Craciunescu, O., Gaspar, A., Trif, M., Moisei, M., Oancea, A., Moldovan, L. and Zarnescu, O. (2014) Preparation and
Characterization of a Collagen-Liposome-Chondroitin Sulfate Matrix with Potential Application for Inflammatory
Disorders Treatment. Journal of Nanomaterials 2014.
9
Del Rey, M. J., Faré, R., Izquierdo, E., Usategui, A., Rodríguez-Fernández, J. L., Suárez-Fueyo, A., Cañete, J. D. and
Pablos, J. L. (2014) Clinicopathological Correlations of Podoplanin (gp38) Expression in Rheumatoid Synovium and Its
Potential Contribution to Fibroblast Plaet Crosstalk. PloS one 9, e99607.
10
Diederichs, S. and Tuan, R. S. (2014) Functional Comparison of Human-Induced Pluripotent Stem Cell-Derived
Mesenchymal Cells and Bone Marrow-Derived Mesenchymal Stromal Cells from the Same Donor. Stem cells and
development.
11
Faulk, D. M., Carruthers, C. A., Warner, H. J., Kramer, C. R., Reing, J. E., Zhang, L., D’Amore, A. and Badylak, S. F.
(2014) The effect of detergents on the basement membrane complex of a biologic scaffold material. Acta biomaterialia
10, 183–193.
12 Feferman, L., Bhattacharyya, S., Birch, L., Prins, G. S. and Tobacman, J. K. (2014) Differential effects of estrogen
exposure on arylsulfatase B, galactose-6-sulfatase, and steroid sulfatase in rat prostate development. The Journal of
steroid biochemistry and molecular biology 143, 105–114.
13
Gilpin, S. E., Guyette, J. P., Gonzalez, G., Ren, X., Asara, J. M., Mathisen, D. J., Vacanti, J. P. and Ott, H. C. (2014)
Perfusion decellularization of human and porcine lungs: Bringing the matrix to clinical scale. The Journal of Heart and
Lung Transplantation 33, 298–308.
14
Gomez-Sanchez, C., Kowalczyk, T., Ruiz De Eguino, G., Lopez-Arraiza, A., Infante, A., Rodriguez, C. I., Kowalewski, T.
A., Sarrionandia, M. and Aurrekoetxea, J. (2014) Electrospinning of poly (lactic acid)/polyhedral oligomeric
silsesquioxane nanocomposites and their potential in chondrogenic tissue regeneration. Journal of Biomaterials
Science, Polymer Edition 1–24.